Fig. 3From: Efficient multiplex mutagenesis by RNA-guided Cas9 and its use in the characterization of regulatory elements in the AGAMOUS geneRecovery of a 5.19 kb deletion in a Cas9-free T2 plant. Detection of mutated allele and the absence of Cas9 T-DNA by PCR (a). The PCR products produced by K1531 and 1532 were analyzed by sequencing (b). The chromatogram of the sequencing result confirms the mutation. T2p#5: plant #5 of a T2 populationBack to article page