The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat

Cápal, Petr; Endo, Takashi R.; Vrána, Jan; Kubaláková, Marie; Karafiátová, Miroslava; Komínková, Eva; Mora-Ramírez, Isabel; Weschke, Winfriede; Doležel, Jaroslav

doi:10.1186/s13007-016-0124-8

Table 2 List of PCR primers for the HOSUT transgene construct and PCR primers for wheat STS markers on chromosomes 1A, 4A, 7A and 5D

From: The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat

Name	Target	Forward primer sequence	Reverse primer sequence	Amplicon size (bp)	Annealing temperature (°C)
HvSUT_6	HOSUT1	AGCGGCGGCGGTCACTGACTG	CCAAAGGACGACACCCCAGCC	265	62
HvSUT_7	HorB1 terminator	ATTAATTCCTCCCCGACCCTGC	CAATGGAGACGGCGCGTGCAA	471	62
HvSUT_11	HOSUT1	GGCGGAACCCGCCGTGCAG	CCTGCGTCTTCCCCATCTGGAAGTA	241	62
HvSUT_RT	HOSUT1	CGGGCGGTCGCAGCTCGCGTCTATT	CATACAGTGACTCTGACCGGCACACA	169	62
Owm121	Chromosome 4A	ATTGCCGTCGCGAACTAGA	CGGGACGAGCTTGACGAT	351	60
Owm126	Chromosome 4A	CCAGTCAGAAATTATTATGAACCTATC	CGCTGTCTCGAGATTGGAGT	342	60
Owm161	Chromosome 4A	TTTTCAAGCAGGTTTTGTGC	TCACTTCTCTTCTTTGCGTTCA	324	60
Owm167	Chromosome 4A	TTTTCTTGGTCAGTATAACCTGTTTTT	TGAGCAGAGAAAAATTTCCAAG	285	60
Owm174	Chromosome 1A	GCATCCTAGTTTCTCTCTCAAGT	AACAAGATCACGAGCGAATTG	157	58
Owm175	Chromosome 1A	AAACCCCTGATACTCATGCG	GTTTCTTGTCATTCATGTCACTTGT	530	58
Owm176	Chromosome 1A	TTCCTGTCTGACTCCGCG	AACCACAACCGTCAACCG	104	58
Owm177	Chromosome 1A	GTAGTCTGCTCCCGAGGAAT	GTCTCTAACCATACATCCATGAAGT	192	58
Owm178	Chromosome 1A	CAACTTCTTCACATCCCGGAA	ATTTGGCCCTATGAGATATAATTACG	306	58
Owm179	Chromosome 1A	ACACTGTGATACCTCTAGATGTATG	CACATTGCCTATAAATTCTAAAAGGTC	425	58
Owm180	Chromosome 5D	CGGACGAGCAGCAGTACC	GCAGATCGGCATAAATTGAATGT	292	58
Owm181	Chromosome 5D	GGAGGTGTTCTAGGTGTACTTACT	AGAGCAATGTCAGAAGTCATCG	240	58
Owm182	Chromosome 5D	TCTCCACCTGCAGAGTCG	CATCAGGCCACAGTGTCAAT	119	58
Owm183	Chromosome 5D	TGTCCACACATTTCCCGTATG	AGTGGTGGATGTGGTTGCT	196	58
Owm184	Chromosome 5D	AGCATGCTCCCAAAGACTATTAC	GTTATGATGGTGGTAGCAATTTGA	400	58
Owm185	Chromosome 5D	GTGAACCTATATGACATCTTACCGG	GGGGCAGTTGTCAAGTATTGC	421	58
Owm186	Chromosome 7A	CTCTCTGTGGCCAATAGTGC	TCTATACCTCAACCCTACATCCA	112	58
Owm187	Chromosome 7A	GGCCACGAATTCCACAAGTA	CTATCGATCAACCAACCATCCA	229	58
Owm188	Chromosome 7A	GTACGAGTGCAGACAGTGTG	ACAATTAATTATACGCCCAGTTAAGC	282	58
Owm189	Chromosome 7A	CGTGCTTTCTTCTTCCTCCG	GCAGGTTAGTTTCTTGTGGTTG	185	58
Owm190	Chromosome 7A	CGCATGGACATTGTTCTAGTCA	GCACTTAGGCACGCTTGAG	517	58
Owm191	Chromosome 7A	CGACGACATTAGGAATATGGGAT	TGCGTGTGGGTGTGCTTA	402	58

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