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Table 1 PCR and FISH analysis of chromosomes sorted from each of the sort gates in three HOSUT lines

From: The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat

Transgenic line Sort gatea PCR result Chromosomes identified FISHc
HOSUT 12/44 Red Negative 4A (92.65 %)
Green Positive 7A (90.90 %)
4A (4.45 %)
HOSUT 20/6 Red Negative 1A (63.75 %)
3D (36.25 %)
Green Positive 5D (94.66 %)
1A (5.33 %)
HOSUT 24/31 Red Semi-positiveb 7A (83.19 %)
4A (12.39 %)
2A (4.42 %)
Green Positive 4A (97.30 %)
  1. aSort gates delineated with green and red rectangles in Fig. 2
  2. bA faint band was visible after agarose gel electrophoresis of PCR product
  3. cMore than 1000 chromosomes were examined in each sorted fraction in each line