Table 1 PCR and FISH analysis of chromosomes sorted from each of the sort gates in three HOSUT lines
From: The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat
Transgenic line | Sort gatea | PCR result | Chromosomes identified FISHc |
|---|---|---|---|
HOSUT 12/44 | Red | Negative | 4A (92.65 %) |
Green | Positive | 7A (90.90 %) | |
4A (4.45 %) | |||
HOSUT 20/6 | Red | Negative | 1A (63.75 %) |
3D (36.25 %) | |||
Green | Positive | 5D (94.66 %) | |
1A (5.33 %) | |||
HOSUT 24/31 | Red | Semi-positiveb | 7A (83.19 %) |
4A (12.39 %) | |||
2A (4.42 %) | |||
Green | Positive | 4A (97.30 %) |