Fig. 5
From: The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat
Agarose gel electrophoresis of PCR products obtained using DNA produced by multiple displacement amplification of three single chromosomes flow-sorted from the sort region representing chromosome 4A in HOSUT 24/31 line. PCR with primers for the four transgenes resulted in products of expected length. The same was true for the chromosome 4A-specific STS markers. Note that none of the chromosome 7A-specific markers was detected in the samples of single chromosome DNA. PCR with genomic DNA of HOSUT 24/31 as template detected both 4A and 7A chromosome-specific markers. PCR with the positive control (represented by 1000 chromosomes sorted from green sorting region and amplified) showed slight PCR bands of chromosome 7A, which reflects a minor contamination of the sorted chromosome 4A by chromosome 7A