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Fig. 3 | Plant Methods

Fig. 3

From: The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat

Fig. 3

Agarose gel electrophoresis of PCR products obtained with primers for the transgene and DNA of chromosomes flow-sorted from three HOSUT lines using the green and red sort regions as shown in Fig. 1. The amplicon of HvSUT-RT (169 bp) was obtained with chromosomes sorted from the green sort region in all three HOSUT lines. When chromosomes were sorted from the red sort regions, no PCR amplification occurred for HOSUT 12/44 and HOSUT 20/6. However, a weak band was observed for HOSUT 24/31. Genomic DNA of the transgenic lines served as positive control

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