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Table 1 An overview of influence of tissue fixation and embedding on RNA quality

From: An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection

Method

Fixation

Cryoprotection in sucrose

Embedding

RNA quality

A

Yes

Yes

Yes (OCT)

Degraded

B

Yes

Yes

No

Degraded

C

Yes

No

No

Good

  1. A, B and C refers to three different steps from which RNA was isolated. (A) RNA was extracted from tissues following all steps of fixation, cryoprotection and embedding, however before LCM. (B) Tissue samples after fixation and cryoprotection but before embedding. (C) Control samples with fixation but without cryoprotection or embedding
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Plant Methods

ISSN: 1746-4811