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Fig. 2 | Plant Methods

Fig. 2

From: An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection

Fig. 2

Quality assessment of RNA isolation from samples after modifying the tissue preparation steps. a RNA isolated after direct incubation in 15 % sucrose solution (low sucrose concentration treated samples). Number of root pieces (containing syncytium) used for extraction: 10; RNA Concentration: 2514 pg/μL. b RNA isolated after direct incubation in 34 % sucrose (high sucrose concentration treated samples). Number of root pieces (containing syncytium) used for extraction: 10; RNA concentration: 2253 pg/μL. c RNA isolation from samples B after LCM. Number of syncytium used for cryosectioning: 30; RNA concentration: 6297 pg/μL. a–c The experiments were repeated three times and data from one representative experiment is provided. S seconds, bp base pair

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Plant Methods

ISSN: 1746-4811