Fig. 1

Influence of tissue fixation and embedding on RNA quality. Electropherograms indicating the quality of RNA extracted from Arabidopsis root segments infected with nematodes at 5 dpi. a RNA was extracted from tissues following all steps of fixation, cryoprotection and embedding, however before LCM. Number of root pieces (containing syncytium) used for extraction: 10; RNA concentration: 3407 pg/μL. b Tissue samples after fixation and cryoprotection but before embedding. There is a splice between Ladder and RNA samples; however, both runs are from the same chip and were put together to facilitate visualization. Number of root pieces (containing syncytium) used for extraction: 15; RNA concentration: 4693 pg/μL. c Control samples with fixation but without cryoprotection or embedding. Number of root pieces (containing syncytium) used for extraction: approx. 25 RNA concentration: 1440 pg/μL. a The experiment was performed once. b, c The experiments were repeated three times and data from one representative experiment is provided. S seconds, bp base pair