Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

Fig. 5

Phloem sap sampling from grafted B. napus plants. 10-week-old grafted plants were punctured with a hypodermic needle below the inflorescence as first described by Giavalisco et al. [28]. The white arrow indicates droplets of phloem sap after puncturing. For phloem sample collection the first droplet is discarded due to the danger of contamination by the content of injured cells. Several hundred microliters of phloem sap could be collected per plant independent on whether they were grafts or not

Back to article page

Plant Methods

ISSN: 1746-4811