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Fig. 3 | Plant Methods

Fig. 3

From: Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

Fig. 3

Post-grafting cultivation of 14 day old B. napus cv. Drakkar grafts. For further cultivation of grafts the plants were transferred to hydroponic culture or soil. For hydroponic cultivation the grafted plants were wrapped in black sponge, in which the graft junction was positioned in the middle and transferred to a 50 ml-Falcon tube consisting 40 ml medium (a). For cultivation on soil grafts were transferred to well-watered soil, in which the graft junction was free of soil (red arrow) and stabilized by a plastic plant label (b). Comparison of graft survival rates after hydroponic and soil cultivation (c). Survivability was determined after 14 days post-cultivation. ½ MS 1 % agar 0.5 % sucrose (hydroponic n = 22; soil n = 23); ½ MS 1 % agar (hydroponic n = 17; soil n = 18); ddH2O (hydroponic n = 18; soil n = 21; 0.5 % sucrose (hydroponic n = 23; soil n = 21)

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