Table 2 Candidate reference gene performance at optimum concentration
Primers | E (%) | R2 | Amplification factor |
|---|---|---|---|
LsUBQ1F/R | 94.9 | 0.992 | 1.95 |
LsUBQ7F/R | 97.3 | 0.945 | 1.97 |
LsACT2F/R | 91.3 | 0.993 | 1.91 |
LsACT12F/R | 94.0 | 0.996 | 1.94 |
LsTUA-3F/R | 90.3 | 0.994 | 1.90 |
LsGAPDHF/R | 91.9 | 0.991 | 1.92 |
LsUBC9F/R | 98.0 | 0.986 | 1.98 |
LsTIP41F/R | 91.6 | 0.996 | 1.92 |
LsPP2AA3F/R | 96.8 | 0.982 | 1.97 |
LsPP2A‐1F/R | 105.9 | 0.920 | 2.06 |