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Fig. 4 | Plant Methods

Fig. 4

From: Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce

Fig. 4

Normalised expression levels of LsFT. a LsFT expression levels normalised using the three most stable reference genes, LsPP2A-1, LsPP2AA3 and LsTIP41, over the diurnal time-course. The white bar below indicates lights on, the black bar indicates darkness. b LsFT expression levels normalised against the two most stable reference genes, LsPP2A-1 and LsTIP41, over the developmental time-course. Samples were collected once a week at 14 h after dawn. The arrow indicates when the plants bolted. c LsFT expression levels normalised against three of the least-stable reference genes, LsUBQ1, LsUBQ7 and LsACT12, over the diurnal time-course. d LsFT expression levels normalised against three of the least-stable reference genes, LsUBQ1, LsUBQ7 and LsACT12, over the developmental time-course

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