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Fig. 5 | Plant Methods

Fig. 5

From: Use of the non-radioactive SUnSET method to detect decreased protein synthesis in proteasome inhibited Arabidopsis roots

Fig. 5

Immunoblot of newly synthesized proteins in roots using SUnSET. Plants were either untreated or treated with MG132 for 4 h, 0.1 M NaCl for 4 days, or grown under sulfur starvation for 4 days. Smaller letters in parenthesis represent band intensity relative to control on dried membranes using image-J. Images are representative of two other experimental replicates. Membranes were stained with Ponceau Red prior to immunblotting

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