Fig. 1

Detection of newly-synthesized proteins containing puromycin in root (a, b) and shoot (c, d) tissue. Cycloheximide treatment (4 h) decreases newly synthesized proteins in plants that were subsequently treated with 20 μM puromycin (b) or 50 μM puromycin (d). Plants were treated with puromycin for 30 min, except in (c). Twenty μg of protein were separated on a 15 % SDS-PAGE gel, and newly synthesized proteins containing puromycin immunoreacted against puromycin antiserum. Membranes were stained with Ponceau Red prior to immunblotting. Smaller letters in parenthesis represent band intensity relative to control on dried membranes using image-J. Images are representative of 3–4 experimental replicates