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Fig. 7 | Plant Methods

Fig. 7

From: Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.

Fig. 7

Images representing leaf infection process. a Mature (approximately 1 week since inoculation) plate of the cacao pathogen Phytophthora palmivora. b Plate of P. palmivora with four agar plugs bored into V8 media. c Inoculation of a new plate of 20 % V8 media by transferring agar plugs, pathogen side down, onto the media. d Typical size of pathogen growth 48 h after inoculation of new plate. Agar plugs are bored around the edges of the cultures to be used for leaf inoculation. e Inoculation of a Stage C cacao leaf with pathogen. Control (media only) plugs are placed on the left side, plugs containing pathogen are placed on the right. f Lesion development 48 h after inoculation. All scale bars represent 1 cm

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