Table 3 Stable transgenic plants expressing RGEN or TALEN constructs
From: A simple test for the cleavage activity of customized endonucleases in plants
Plant species | Target gene | Type of customized endonuclease | PCR positive | Mutant plants | Ratio PCR+/mutants (%) | |
|---|---|---|---|---|---|---|
Cas9/FokI | gRNA | |||||
Tobacco | gfp | RGEN | 17 | 15 | 12 | 80.0 |
TALEN | 35 | n.a. | 1 | 2.9 | ||
Barley | gfp | TALEN | 66 | n.a. | 4 | 6.1 |
MLO | TALEN | 6 | n.a. | 3 | 50.0 | |