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Table 3 Stable transgenic plants expressing RGEN or TALEN constructs

From: A simple test for the cleavage activity of customized endonucleases in plants

Plant species Target gene Type of customized endonuclease PCR positive Mutant plants Ratio PCR+/mutants (%)
Cas9/FokI gRNA
Tobacco gfp RGEN 17 15 12 80.0
TALEN 35 n.a. 1 2.9
Barley gfp TALEN 66 n.a. 4 6.1
MLO TALEN 6 n.a. 3 50.0
  1. n.a. not analysed