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Table 2 Relative cleavage activity of RGEN and TALEN constructs in transiently transformed barley and tobacco leaf explants

From: A simple test for the cleavage activity of customized endonucleases in plants

Plant species Target gene Type of customized endonuclease Constructs used Experiment YFP cells mCherry cells Ratio YFP/mCherry cells (%)
Tobacco gfp RGEN pTARGET-gfp1 1 273 371 73.6
+ RGEN-gfp 2 342 389 87.9
  3 324 499 64.9
  Average 313 ± 29 420 ± 57 74.6
pTARGET-gfp1 1 0 106 0
  2 0 204 0
  3 0 81 0
  Average 0 130 ± 65 0
TALEN pTARGET-gfp1 1 195 361 54.0
+ TALEN-gfp 2 25 183 13.7
  3 80 545 14.7
  Average 100 ± 71 363 ± 148 27.5
Barley gfp RGEN pTARGET-gfp2 1 72 207 34.8
+ RGEN-gfp 2 55 206 26.7
  3 83 255 32.5
  Average 70 ± 12 223 ± 23 31.4
pTARGET-gfp2 1 0 46 0
TALEN pTARGET-gfp1 1 71 223 31.8
+ TALEN-gfp 2 110 331 33.2
  3 49 195 25.1
  Average 77 ± 25 250 ± 59 30.7
pTARGET-gfp1 1 0 44 0
MLO TALEN pTARGET-MLO 1 134 350 38.3
+ TALEN-MLO 2 107 254 42.1
  3 112 237 47.3
  Average 118 ± 12 280 ± 50 42.0
pTARGET-MLO 1 0 52 0