Fig. 5
From: A simple test for the cleavage activity of customized endonucleases in plants
The structure of the customized endonuclease constructs. AtUBI10P-int: A. thaliana UBIQUITIN-10 promoter with intron; gfp-BD-L/R: synthetic S65T green fluorescent protein left/right TALEN binding domain; FokI: FokI cleavage domain; OCST: A. tumefaciens OCS terminator; PcUBI4-2P: Petroselinum crispum UBIQUITIN4-2 promoter; aCas9: A. thaliana codon-optimzed Cas9; Ps3AT: pea Pea3A terminator; AtU6-26P: A. thaliana U6-26 promoter; gRNA: guide-RNA; ZmUBI1P-int: maize UBIQUITIN1 promoter with first intron; NOST: NOS terminator; NLS: SV40 Simian virus 40 nuclear localization signal; 3xFLAG tag: multimeric synthetic FLAG octapeptide; HA tag: hemagglutinin tag; N-term: N-terminus of a modified version of Xanthomonas campestris pv. vesicatoria AvrBs3; C-term: C-terminus of a modified version of AvrBs3. The elements shown are not drawn to scale