Fig. 4

Alignment of mutated MLO sequences recovered from three barley transformants (1E01, 1E03, and 2E02) induced by the presence of a TALEN construct. MLO-specific PCR products were subcloned and up to ten clones were sequenced. The sequences shown in red and blue represent, respectively the left and right TALEN binding sites; the number of nucleotides deleted (dashes) and the number of mutants/number of clones analysed is shown to the right of each sequence