Fig. 5

Simultaneous RNAi mediated down-regulation of expression of six target genes in flowers of Arabidopsis hpRNAi lines transformed with a single RNAi construct. Four Arabidopsis T2 lines transformed with pAGRIKOLA-LNTMHS, two T2 lines transformed with pAGRIKOLA-HTLNSM, and one T2 line transformed with pAGRIKOLA-NLSHMT were tested for each of the target genes, AtHY2, AtTRY, AtLNG1, AtNPQ1, AtSEX1 and AtMAX3. Abundance of the respective target transcript are given as relative expression \((2^{{ - \Delta \Delta {\text{C}}_{\text{T}} }} )\) compared to that of an Arabidopsis line expressing a pAGRIKOLA-Control RNAi fragment and AtACT2 for normalization