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Fig. 4 | Plant Methods

Fig. 4

From: Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Fig. 4

RNAi mediated down-regulation of target gene expression in single Arabidopsis hpRNAi lines. T2 individuals of three individual pAGRIKOLA hpRNAi expressing Arabidopsis lines were tested for each of the target genes, AtHY2, AtTRY, AtLNG1, AtNPQ1, AtSEX1 and AtMAX3. Abundance of the respective target transcript are given as relative expression \((2^{{ - \Delta \Delta {\text{C}}_{\text{T}} }} )\) compared to that of an Arabidopsis line expressing a pAGRIKOLA-Control RNAi fragment and AtACT2 for normalization

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