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Fig. 3 | Plant Methods

Fig. 3

From: Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Fig. 3

Series of vectors created to clone the HTLNSM synthetic multiple RNAi fragment. a Gene specific tags for AtHY2, AtTRY, AtLNG1, AtNPQ1, AtSEX1 and AtMAX3 were assembled to form the HTLNSM multiple RNAi fragment in yeast. b The HTLNSM synthetic DNA fragment was amplified by PCR using pYES1L-HTLNSM as template and the PCR product was cloned into pENTR™/D-TOPO to create pENTR-HTLNSM. The CACC-overhang was introduced to facilitate directional cloning into pENTR™/D-TOPO. c The HTLNSM synthetic DNA fragment was subsequently transferred to pAGRIKOLA by LR recombination reaction to create the hpRNAi vector pAGRIKOLA-HTLNSM. Refer to Thermo Fisher Scientific Inc., (www.thermofisher.com) for more information about pYES1L and pENTRY and to Hilson et al. [20] for pAGRIKOLA

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