Fig. 2

Synthetic multiple RNAi fragments assembled in pYES1L from double-stranded PCR products by a single yeast transformation event. Primers used to amplify individual gene specific tags (GSTs) are given in yellow. Synthetic fragments (a–c) contain six different GSTs in different orders, while fragments (d–f) carry an additional GST for the gene GUN4. Total fragment lengths are 1534 bp for the six-gene and 1934 bp for the seven-gene multiple RNAi fragment. PstI restriction sites are labeled by red arrows