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Fig. 5 | Plant Methods

Fig. 5

From: A method for detecting single mRNA molecules in Arabidopsis thaliana

Fig. 5

Automated image analysis of PP2A mRNA. a Representative maximum projection image of cell files labeled with PP2A mRNA probes (red). DNA labeled with DAPI (blue). b, c Screen shots showing sequential detection steps used to determine positive mRNA signals. d Cell segmentation, where a false-color is rendering individual cells. e Output image indicating the number of mRNA signals detected on each cell segmented in (d). Scale bar = 10 μm

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