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Table 1 Phytohormone profiles are less affected by the attBSMV:00 compared to BSMV:GFP infection

From: Identification of an attenuated barley stripe mosaic virus for the virus-induced gene silencing of pathogenesis-related wheat genes

 

Uninfected (ng/g DW)

Fusarium infected (ng/g DW)

ABA

 WT

154 ± 31.0

227 ± 62.7

 BSMV:GFP

193 ± 29.5

458 ± 54.2##, *

 attBSMV:00

144 ± 23.8

346 ± 122*

Dihydrophaseic acid

 WT

28.2 ± 37.4

71.4 ± 64.7

 BSMV:GFP

20.5 ± 7.8

916 ± 96.7####, ****

 attBSMV:00

31.1 ± 7.2

52.7 ± 19.8

Phaseic acid

 

 WT

112.5 ± 9.3

213 ± 98.4

 BSMV:GFP

84.0 ± 38.7

678 ± 353#, **

 attBSMV:00

65.8 ± 28.1

83.4 ± 22.0

 

Uninfected (ng/g WW)

F. graminearum (ng/g WW)

JA

 WT

22.8 ± 3.4

63.7 ± 12.1

 BSMV:GFP

27.1 ± 7.9

506.0 ± 105.6####, ****

 attBSMV:00

15.8 ± 3.8

40.8 ± 6.8

JA-Isoleucine

 WT

10.9 ± 4.6

20.3 ± 3.9

 BSMV:GFP

7.6 ± 2.1

174.9 ± 31.4 ####, ****

 attBSMV:00

8.4 ± 2.8

16.0 ± 6.9

SA

 WT

121.8 ± 32.3

137.1 ± 56.4

 BSMV:GFP

260.6 ± 67.6

835.3 ± 152.4####, ****

 attBSMV:00

96.2 ± 23.6

91.8 ± 27.9

Conjugated SA

 WT

1121.9 ± 77.0

681.7 ± 49.0**

 BSMV:GFP

657.8 ± 246.2##

786.1 ± 65.3

 attBSMV:00

415.5 ± 86.8####

228.3 ± 16.2##

  1. Values represent the average of three biological ‘Fielder’ spike replicates with standard deviation. ABA and its catabolites, dihydrophaseic and phaseic acids, were quantified from lyophilized tissue [dry weight (DW)]; while JA, SA, and metabolites were quantified without a lyophilization step [wet weight (WW)]. Differences upon BSMV (denoted by ‘#’) or Fusarium infection (denoted with ‘*’) were evaluated by two-way ANOVA with Sidak post hoc comparisons (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001)
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Plant Methods

ISSN: 1746-4811