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Fig. 1 | Plant Methods

Fig. 1

From: Identification of an attenuated barley stripe mosaic virus for the virus-induced gene silencing of pathogenesis-related wheat genes

Fig. 1

BSMV and Fusarium co-infection result in nonviable ‘Fielder’ spikes, increased DON contamination, and reduced yield. a WT, Fusarium-infected, BSMV:GFP-infected, BSMV:GFP and Fusarium co-infected, BSMV:00-infected, and BSMV:00 and Fusarium co-infected ‘Fielder’ spikes (from left to right) at 10 days post Fusarium inoculation. b Necrotic ‘Fielder’ spikes are averages of three replicate experiments, each composed of a minimum of 25 spikes, with standard error. c From one representative experiment, DON contamination was determined in five biological replicate spikes with standard deviation at 5 day intervals over the course of the infection. d Grains from non-BSMV, BSMV:GFP, and BSMV:00 infected tissue (left to right) without (top) and with (bottom) secondary Fusarium infection. e Yield represents the average of three biological replicate grain samples, sufficient for thousand grain weight calculation, from a representative experiment with standard deviation. ‘Uninfected’ on the x-axis refers to BSMV. ‘Uninfected’ in the inserted legend refers to Fusarium. f From one representative experiment, DON contamination was evaluated in three biological replicate grain samples with standard deviation. ‘Uninfected’ on the x-axis refers to BSMV. Differences in spike necrosis and DON contamination were analyzed with one-way ANOVA with Dunnett post hoc comparisons; while yield differences were evaluated with two-way ANOVA with Sidak post hoc comparisons. Changes between upon BSMV-infection are denoted with ‘#’ while Fusarium infected are denoted with ‘*’ (*p ≤ 0.05; ***p ≤ 0.001; ****p ≤ 0.0001)

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