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Fig. 2 | Plant Methods

Fig. 2

From: Gene targeting and transgene stacking using intra genomic homologous recombination in plants

Fig. 2

Crossing and targeted plant production strategy in maize using intra-genomic homologous recombination. Plants homozygous to donor (a) and target (b) are crossed and self-pollinated to obtain progenies that are homozygous to target and donor loci (c). The homozygous target donor plants are crossed with plants containing designed nuclease (DN) transgene (d) to obtain F1 progenies transgenic to target, donor and DN (e). The F1 immature embryos are treated on appropriate selection media (f) and targeted plants are regenerated on selection (g and h). Alternatively, the F1 plants could be selection sprayed (i) to obtain targeted plants (j)

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