Fig. 6

Direct comparison of cuvette-luminometer and micro titre plate luminometer based methods. a, b Oxidative burst in Oryza sativa suspension cultured cells using L012 as a chemiluminescent reagent in a 96 well plate elicited with different concentrations of chitosan (0.01–0.33 mg/ml) (data shown are means of triplicates). During the 120-min measuring period, a first peak with high amplitude and short duration was observed followed by a second peak with longer duration (a). Each peak maximum was used to plot dose-response curves, in which peak 1 is defined as 0–20 min and peak 2 as 25–100 min (b). c, d Oxidative burst in O. sativa suspension cultured cells using luminol as a chemiluminescent reagent in a cuvette, with different concentrations of chitosan as an elicitor (0.01–0.35 mg/ml). During the 120-min measuring period, a peak reached around 90 min was preceded by a shoulder which was defined as “peak 1” (c). Dose-response curves were plotted, in which peak 1 is defined as 0–20 min and peak 2 as 25–100 min (d). All plots represent one out of three independent experiments which gave similar results