Fig. 3From: An improved microtiter plate assay to monitor the oxidative burst in monocot and dicot plant cell suspension culturesPriming dose dependency in the dicot Medicago truncatula. Dose dependency of the oxidative burst in Medicago truncatula cell suspension cultures pre-treated with different concentrations of salicylic acid (0.8–9.6 µM) in triplicates, then elicited with ulvan (0.08 mg/ml). During the 90-min measurement period, two peaks can be observed (a). The first peak maximum (0–25 min) was used to plot a dose dependency curve (b). The curve for the second peak was similar (not shown). The data shown in a are taken from one experiment which is representative of three independent experiments. The data shown in b are mean values ± SD of these three experiments with the maximum relative light units (RLUs) reached in each experiment set to 100 %Back to article page