TY - JOUR AU - Visendi, Paul AU - Berkman, Paul J. AU - Hayashi, Satomi AU - Golicz, Agnieszka A. AU - Bayer, Philipp E. AU - Ruperao, Pradeep AU - Hurgobin, Bhavna AU - Montenegro, Juan AU - Chan, Chon-Kit Kenneth AU - Staňková, Helena AU - Batley, Jacqueline AU - Šimková, Hana AU - Doležel, Jaroslav AU - Edwards, David PY - 2016 DA - 2016/01/20 TI - An efficient approach to BAC based assembly of complex genomes JO - Plant Methods SP - 2 VL - 12 IS - 1 AB - There has been an exponential growth in the number of genome sequencing projects since the introduction of next generation DNA sequencing technologies. Genome projects have increasingly involved assembly of whole genome data which produces inferior assemblies compared to traditional Sanger sequencing of genomic fragments cloned into bacterial artificial chromosomes (BACs). While whole genome shotgun sequencing using next generation sequencing (NGS) is relatively fast and inexpensive, this method is extremely challenging for highly complex genomes, where polyploidy or high repeat content confounds accurate assembly, or where a highly accurate ‘gold’ reference is required. Several attempts have been made to improve genome sequencing approaches by incorporating NGS methods, to variable success. SN - 1746-4811 UR - https://doi.org/10.1186/s13007-016-0107-9 DO - 10.1186/s13007-016-0107-9 ID - Visendi2016 ER -