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Table 1 Promoters tested for cell type enriched expression in L. japonicus roots

From: Inoculation insensitive promoters for cell type enriched gene expression in legume roots and nodules

Predominant expression in L. japonicus roots Promoter name Promoter fragment used (bp) Sequence origin (species) Locus/accession Gene product References
Epidermis (trichoblast/atrichoblast) pLeExt1 a,b 1122 Tomato NM_001247899 Extensin/xyloglucanv endotransglycosylase Bucher et al. [32], Mirabella et al. [33]
Epidermis and cortex pAtS31 3041 Arabidopsis At5g19790 ERF/AP2 TF Lee et al. [6]
pAtRHS14 1249 Arabidopsis At4g22080 Pectin lyase Won et al. [35]
Cortex pAtCortex a 1666 Arabidopsis At1g09750 Aspartyl protease Lee et al. [6], Dinneney et al. [4]
Endodermis and pericycle pAtE29 a 2724 Arabidopsis At4g05170 bHLH TF Lee et al. [6]
pAtE47 3296 Arabidopsis At2g37950 C3H TF Lee et al. [6]
Pericycle and phloem pAtS5 2202 Arabidopsis At5g24800 bZIP TF Lee et al. [6]
Phloem and pericycle pAtS32 3000 Arabidopsis At2g18380 C2C2-Gata TF Lee et al. [6]
pAtS13 a 4018 Arabidopsis At1g07640 Dof TF Lee et al. [6]
pAtSUC2 c 942 Arabidopsis At1g22710 Sucrose-proton symporter Stadler et al. [37]
Proto- and metaxylem pAtS18 a 3010 Arabidopsis At5g12870 MYB TF Lee et al. [6]
Unspecific pLjPDC1 2987 L. japonicus Lj1g2372300 Pyruvate decarboxylase This manuscript
  pAtE30 3004 Arabidopsis At4g21340 bHLH TF Lee et al. [6]
  pAtE31 1484 Arabidopsis At4g28890 C3H TF Lee et al. [6]
  pAtE49 d 2414 Arabidopsis At3g05150 C2H2 TF Lee et al. [6]
Unstable (phloem) pAtS8 e 3082 Arabidopsis At5g60200 C2C2-Dof TF Lee et al. [6]
Unstable (proto- and metaxylem) pAtS20 f 3002 Arabidopsis At1g71930 NAC TF Lee et al. [6]
  1. Where promoter activity was detected in more than one cell type, the cell type of primary expression is underlined. Arabidopsis, A. thaliana; tomato, Lycopersicon esculentum; TF, transcription factor
  2. aAvailable in transgenic lines expressing GUS
  3. bFor pLeExt1, expression is enhanced in trichoblasts as compared to atrichoblasts
  4. cExpression of pAtSUC2 was exclusively seen in roots containing chloroplasts due to exposure to light
  5. d pAtE49 shows unstable expression across most root cell types
  6. eExpression of pAtS8 is primarily associated with phloem poles, but occasional unspecific expression across root cell types is observed
  7. f pAtS20 showed rare xylem associated expression. Where promoter activity was detected in more than one cell type, the cell type of primary expression intensity is underlined