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Table 1 Promoters tested for cell type enriched expression in L. japonicus roots

From: Inoculation insensitive promoters for cell type enriched gene expression in legume roots and nodules

Predominant expression in L. japonicus roots

Promoter name

Promoter fragment used (bp)

Sequence origin (species)

Locus/accession

Gene product

References

Epidermis (trichoblast/atrichoblast)

pLeExt1 a,b

1122

Tomato

NM_001247899

Extensin/xyloglucanv endotransglycosylase

Bucher et al. [32], Mirabella et al. [33]

Epidermis and cortex

pAtS31

3041

Arabidopsis

At5g19790

ERF/AP2 TF

Lee et al. [6]

pAtRHS14

1249

Arabidopsis

At4g22080

Pectin lyase

Won et al. [35]

Cortex

pAtCortex a

1666

Arabidopsis

At1g09750

Aspartyl protease

Lee et al. [6], Dinneney et al. [4]

Endodermis and pericycle

pAtE29 a

2724

Arabidopsis

At4g05170

bHLH TF

Lee et al. [6]

pAtE47

3296

Arabidopsis

At2g37950

C3H TF

Lee et al. [6]

Pericycle and phloem

pAtS5

2202

Arabidopsis

At5g24800

bZIP TF

Lee et al. [6]

Phloem and pericycle

pAtS32

3000

Arabidopsis

At2g18380

C2C2-Gata TF

Lee et al. [6]

pAtS13 a

4018

Arabidopsis

At1g07640

Dof TF

Lee et al. [6]

pAtSUC2 c

942

Arabidopsis

At1g22710

Sucrose-proton symporter

Stadler et al. [37]

Proto- and metaxylem

pAtS18 a

3010

Arabidopsis

At5g12870

MYB TF

Lee et al. [6]

Unspecific

pLjPDC1

2987

L. japonicus

Lj1g2372300

Pyruvate decarboxylase

This manuscript

 

pAtE30

3004

Arabidopsis

At4g21340

bHLH TF

Lee et al. [6]

 

pAtE31

1484

Arabidopsis

At4g28890

C3H TF

Lee et al. [6]

 

pAtE49 d

2414

Arabidopsis

At3g05150

C2H2 TF

Lee et al. [6]

Unstable (phloem)

pAtS8 e

3082

Arabidopsis

At5g60200

C2C2-Dof TF

Lee et al. [6]

Unstable (proto- and metaxylem)

pAtS20 f

3002

Arabidopsis

At1g71930

NAC TF

Lee et al. [6]

  1. Where promoter activity was detected in more than one cell type, the cell type of primary expression is underlined. Arabidopsis, A. thaliana; tomato, Lycopersicon esculentum; TF, transcription factor
  2. aAvailable in transgenic lines expressing GUS
  3. bFor pLeExt1, expression is enhanced in trichoblasts as compared to atrichoblasts
  4. cExpression of pAtSUC2 was exclusively seen in roots containing chloroplasts due to exposure to light
  5. d pAtE49 shows unstable expression across most root cell types
  6. eExpression of pAtS8 is primarily associated with phloem poles, but occasional unspecific expression across root cell types is observed
  7. f pAtS20 showed rare xylem associated expression. Where promoter activity was detected in more than one cell type, the cell type of primary expression intensity is underlined