Fig. 2
From: Inoculation insensitive promoters for cell type enriched gene expression in legume roots and nodules
Patterns of cell type enriched promoter activity as indicated by GUS staining are maintained following whole plant regeneration from calli using selected promoter:GUS constructs. Transgenes are a–c pLeExt1:GUS (line LIG29-2); d–f pAtCortex:GUS (line CoG11-6); g–i pAtE29:GUS (line 29G4b-5); j–l pAtS13:GUS (line 13G26b-28) and m–o pAtS18:GUS (line XG5A-5). Uninoculated transgenic plants were harvested 3 weeks post germination. Representative GUS stained root tips (a, d, g, j, m entire root mounts) and responsive zone fragments (b, e, h, k, n entire root mounts; c, f, i, l, o cross sections) are shown. Cross sections are 7–8 µm microtome sections of resin (Kulzer Technovit 7100) embedded roots stained with 0.1 % Ruthenium Red. Scale bars 50 µm