Table 1 Published standard parts for plants for use in the assembly of binary plasmid vectors for Cas9-mediated targeted mutagenesis

Constitutive promoters (PROM) and strong 5′ untranslated regions (5UTR) for use in Cas9 and selectable markers gene cassettes

 pICH51266

PROM (1.3 kb), 35s (Cauliflower Mosaic Virus) + 5UTR omega (Tobacco Mosaic Virus)

#50267

[20]

 pICH51288

PROM (double), 35s (Cauliflower Mosaic Virus) + 5UTR, omega (Tobacco Mosaic Virus)

#50269

[20]

 pICSL12009

PROM and 5UTR Ubiquitin (Zea mays)

#68257

[22]

 pICH87633

PROM, nopoline syntase (Agrobacterium tumefaciens) + 5UTR, omega (Tobacco Mosaic Virus)

#50271

[20]

 pICH85281

PROM + 5UTR, mannopine synthase (A. tumefaciens) + 5UTR, omega (Tobacco Mosaic Virus)

#50272

[20]

 pICSL12006

PROM + 5UTR (Cassava Vein Mosaic Virus)

#50270

[20]

Small RNA promoters for single guide RNAs

 pICSL9003

PROM U6 (Triticum aestivum)

#68262

[22]

 pICSL90002

PROM U6-26 (Arabidopsis thaliana)

#68261

[22]

Coding sequences (CDS) of selectable markers genes

 pICSL80037

CDS neomycin phosphotransferase II (Escherichia coli)

#68260

[22]

 pICSL80036

CDS hygromycin phophotransferase II (E. coli)

#68259

[22]

 piCH42222

Phosphinothricin acetyl transferase, (Streptomyces hygroscopicus)

#50328

[20]

 pICH43844

CDS phosphinothricin acetyl transferase, (S. hygroscopicus) with intron from ACT2 (A. thaliana)

#50329

[20]

3′ untranslated regions (3UTR) and polyadenylation signals (TERM) for use in Cas9 and selectable markers gene cassettes

 pICH41414

3UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

#50337

[20]

 pICH41421

3UTR + TERM, nopaline synthase (A. tumefaciens)

#50339

[20]

 pICH41432

3UTR + TERM octopine synthase (A. tumefaciens)

#50343

[20]

 piCH72400

3UTR + TERM g7 (A. tumefaciens)

#50338

[20]

Cas9 coding sequence and single guide RNA scaffold

 pICH41308 ::hCas9

CDS Cas9 (Streptococcus pyogenes)

#49770

[23]

 pICSL11061

Single guide RNA scaffold

#46966

[24]

Assembled selectable marker and Cas9 transcriptional units

 pICSL11055

pICH51288 (double 35s) + pICH80037 (nptII) + pICH41421 (nos) in pICH47732 (Level 1 Position 1) backbone

#68252

[22]

 pICSL11059

pICH51266 (35s) + PICSL80036 (hptII) + pICH41414 (35 s) in pICH47732 (Level 1 Position 1) backbone

#68263

[22]

 pICSL11060

pICSL12006 (CsVMV) + pICH41308::hCas9 (Cas9) + pICH41414 (35s) in pICH47742 (Level 1 Position 2) backbone

#68264

[22]

 pICSL11056

pICSL12009 (ZmUbi) + pICH41308::hCas9 (Cas9) + pICH41421(nos) in pICH47742 (Level 1 Position 2) backbone

#68258

[22]

 pICH47742::2x35S-5′UTR-hCas9(STOP)-NOST

pICH51288 (double 35 s) + pICH41308::hCas9 (Cas9) + pICH41421(nos) in pICH47742 (Level 1 Position 2) backbone

#49771

[23]