Constitutive promoters (PROM) and strong 5′ untranslated regions (5UTR) for use in Cas9 and selectable markers gene cassettes
|
pICH51266
|
PROM (1.3 kb), 35s (Cauliflower Mosaic Virus) + 5UTR omega (Tobacco Mosaic Virus)
|
#50267
|
[20]
|
pICH51288
|
PROM (double), 35s (Cauliflower Mosaic Virus) + 5UTR, omega (Tobacco Mosaic Virus)
|
#50269
|
[20]
|
pICSL12009
|
PROM and 5UTR Ubiquitin (Zea mays)
|
#68257
|
[22]
|
pICH87633
|
PROM, nopoline syntase (Agrobacterium tumefaciens) + 5UTR, omega (Tobacco Mosaic Virus)
|
#50271
|
[20]
|
pICH85281
|
PROM + 5UTR, mannopine synthase (A. tumefaciens) + 5UTR, omega (Tobacco Mosaic Virus)
|
#50272
|
[20]
|
pICSL12006
|
PROM + 5UTR (Cassava Vein Mosaic Virus)
|
#50270
|
[20]
|
Small RNA promoters for single guide RNAs
|
pICSL9003
|
PROM U6 (Triticum aestivum)
|
#68262
|
[22]
|
pICSL90002
|
PROM U6-26 (Arabidopsis thaliana)
|
#68261
|
[22]
|
Coding sequences (CDS) of selectable markers genes
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pICSL80037
|
CDS neomycin phosphotransferase II (Escherichia coli)
|
#68260
|
[22]
|
pICSL80036
|
CDS hygromycin phophotransferase II (E. coli)
|
#68259
|
[22]
|
piCH42222
|
Phosphinothricin acetyl transferase, (Streptomyces hygroscopicus)
|
#50328
|
[20]
|
pICH43844
|
CDS phosphinothricin acetyl transferase, (S. hygroscopicus) with intron from ACT2 (A. thaliana)
|
#50329
|
[20]
|
3′ untranslated regions (3UTR) and polyadenylation signals (TERM) for use in Cas9 and selectable markers gene cassettes
|
pICH41414
|
3UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)
|
#50337
|
[20]
|
pICH41421
|
3UTR + TERM, nopaline synthase (A. tumefaciens)
|
#50339
|
[20]
|
pICH41432
|
3UTR + TERM octopine synthase (A. tumefaciens)
|
#50343
|
[20]
|
piCH72400
|
3UTR + TERM g7 (A. tumefaciens)
|
#50338
|
[20]
|
Cas9 coding sequence and single guide RNA scaffold
|
pICH41308 ::hCas9
|
CDS Cas9 (Streptococcus pyogenes)
|
#49770
|
[23]
|
pICSL11061
|
Single guide RNA scaffold
|
#46966
|
[24]
|
Assembled selectable marker and Cas9 transcriptional units
|
pICSL11055
|
pICH51288 (double 35s) + pICH80037 (nptII) + pICH41421 (nos) in pICH47732 (Level 1 Position 1) backbone
|
#68252
|
[22]
|
pICSL11059
|
pICH51266 (35s) + PICSL80036 (hptII) + pICH41414 (35 s) in pICH47732 (Level 1 Position 1) backbone
|
#68263
|
[22]
|
pICSL11060
|
pICSL12006 (CsVMV) + pICH41308::hCas9 (Cas9) + pICH41414 (35s) in pICH47742 (Level 1 Position 2) backbone
|
#68264
|
[22]
|
pICSL11056
|
pICSL12009 (ZmUbi) + pICH41308::hCas9 (Cas9) + pICH41421(nos) in pICH47742 (Level 1 Position 2) backbone
|
#68258
|
[22]
|
pICH47742::2x35S-5′UTR-hCas9(STOP)-NOST
|
pICH51288 (double 35 s) + pICH41308::hCas9 (Cas9) + pICH41421(nos) in pICH47742 (Level 1 Position 2) backbone
|
#49771
|
[23]
|