Fig. 3From: Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/CasOverview of the Cas9-mediated in planta GT system. The nuclease and the DNA donor sequence are located on one T-DNA that is stably transformed into the plant. The nuclease induces two DSBs that release the donor intermediate and a third DSB that activates the target locus for HR. The donor sequence integrates into the target locus by using the flanking homologous regionsBack to article page