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Table 2 Comparison of three different two-choice assays to acquire data on plant resistance to thrips

From: Automated video tracking of thrips behavior to assess host-plant resistance in multiple parallel two-choice setups

  Whole plants Detached leaves Video tracking
Main advantages Non-invasive
Reproduction and survival data
Limited space and number of insects required
More standardized setup (allows automated imaging)
Detailed behavioral parameters
Quick and objective
controlled conditions
Main disadvantages Space consuming
Large numbers of thrips required
Time consuming analysis
large environmental variation
Limited mechanical damage at petiole
Senescence of material
Time consuming analysis
Mechanical damage at edge of leaf disc
Relationship to endpoint values unknown
Variables obtained Feeding damage
Feeding damage Duration spent in zone
Duration not moving
Duration moving
Ratio moving/not moving
Distance moved
Inoculation 30 min 60 min 30 min
Duration 6 days 6 days 8 h
Analysis 2–4 h 1 h 10 min
Minimum number of replicatesa 5 5 15
# thrips required 100 (5 × 20) 10 (5 × 2) 15 (15 × 1)
  1. aThe minimum number of replications is based on the criterion that >50 % of experiments should be significantly different among genotypes [P < 0.05 (Additional file 2: Table S1)]