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Table 1 Results of three different dual-choice setups for testing thrips preference on two Arabidopsis accessions

From: Automated video tracking of thrips behavior to assess host-plant resistance in multiple parallel two-choice setups

Variable

Cur-3 resistant

Rmx-A180 susceptible

Video assaya (total 8 h)

  

 Duration spent in zone (s)

5026 ± 470

8292 ± 631**

 Duration not moving (s)

4122 ± 446

7494 ± 632***

 Duration moving (s)

895 ± 80

787 ± 73*

 Activity ratio (mov/not mov) (%)

22 ± 2 %

11 ± 2 %***

 Distance moved (mm)

870 ± 70

926 ± 68

 Movement velocity (mm/s)

0.65 ± 0.02

0.68 ± 0.02

Leaf assayb

  

 Damage after 6 days (mm2)

8.5 ± 2

45.6 ± 5***

Plant assayc

  

 Damage after 6 days (mm2)

25 ± 5.5

837 ± 62.3**

 # of nymphs after 7 days

2.2 ± 0.2

8.7 ± 1***

  1. * P < 0.05; ** P < 0.01; *** P < 0.001, Wilcoxon signed-rank test
  2. aBehavior of thrips was monitored for eight hours (one adult female thrips per arena, N = 68, details of parameter settings in the methods section)
  3. bFeeding damage was estimated after six days (two thrips per arena, N = 24)
  4. cFeeding damage after six days was scored. In addition, the number of emerged nymphs was scored from the original inoculation of 20 adult female thrips per arena, N = 9). All assays used female adults of approximately 3 weeks old
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Plant Methods

ISSN: 1746-4811