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Table 1 Results of the quantification and quality analyses of mtDNA extracted from different seed and leaf tissues of five different plant species

From: An improved method with a wider applicability to isolate plant mitochondria for mtDNA extraction

Sample

PicoGreen ng/µl

NanoDrop ng/µl

A260/A280

A260/A230

AC Barrie

34.33

625

1.83

2.08

Superb

33.88

606

1.88

2.09

Dead seed B

27.02

410

1.94

1.93

Dead seed S

27.43

413

2.01

2.00

AS B

29.69

530

1.91

2.00

AS S

30.93

513

1.97

2.08

AL B

28.83

493

1.90

2.02

AL S

28.33

491

1.95

2.09

NS B

29.57

634

1.83

2.03

NS S

27.88

620

1.85

2.06

NL B

27.22

471

1.92

2.05

NL S

26.16

485

1.84

2.03

Oat

34.38

617

1.94

2.04

Arabidopsis

30.62

544

1.89

2.11

Mustard

30.19

484

1.85

2.00

Flax

31.28

494

1.93

1.98

  1. A mtDNA sample was measured for both quantity and quality by NanoDrop, diluted to 40 ng/µl and confirmed for quantity by PicoGreen
  2. AS abnormally germinated seed tissue, AL abnormally germinated leaf tissue, NS normally germinated seed tissue, NL normally germinated leaf tissue
  3. Sample names with B or S represent wheat genotypes AC Barrie or Superb, respectively