Fig. 3

Integrity and purity tests of mtDNA extracted from five seed and leaf tissues of two wheat genotypes. For integrity test ~28 µg of purified mtDNA was loaded on to the gel, showing in Template. For purity test 120 ng of mtDNA was used for each PCR reaction with specific mitochondria, chloroplast and nuclear primers (COX1, RBCL and actin, respectively). −VE indicates PCR with no template with the actin primers. +VE indicates unpurified mtDNA used as template with three different sets of primers. The purity based on three specific primer sets is also shown. To illustrate, black arrow head is also used to show mtDNA stacked in the wells; black arrow, intact mtDNA; a green arrow, degraded mtDNA; white arrow heads, target products with +VE controls; white arrows, target product with COX1; red arrows, target product with RBCL and actin; blue arrows, target amplification from residual template with RBCL or actin primers. Names of the seed and leaf tissues corresponding to each reaction are represented at the top, and DNA ladder in kb is represented on the left side of the gel. AS abnormally germinated seed tissue, AL abnormally germinated leaf tissue, NS normally germinated seed tissue, NL normally germinated leaf tissue. Sample names with B or S represent wheat genotypes AC Barrie or Superb, respectively