Table 1 Combinations of staining temperature and pre-staining incubation time tested to determine best conditions to differentiate fungal structures when staining wheat leaf specimens inoculated with P. triticina
From: Rapid protocol for visualization of rust fungi structures using fluorochrome Uvitex 2B
Staining temperature | Room temperature (min) | 65 °C (min) |
|---|---|---|
Pre-staining incubation time | 0 | 0 |
5 | 5 | |
10 | 10 | |
15 | 15 | |
30 | 30 |