Fig. 5
From: Rapid protocol for visualization of rust fungi structures using fluorochrome Uvitex 2B
Sample preparation for laser capture microdissection of infected wheat cells. Wheat leaf specimens examined under an Axio Imager M2 Research epifluorescence microscope (a, b). a Longitudinal section of non-inoculated wheat leaf stained with Uvitex 2B. b Longitudinal section of wheat leaf inoculated with P. triticina. Within box, infection site with uredinium was excised via LCM and collected for RNA extraction of host and pathogen cells