Fig. 3

Puccinia triticina infection on susceptible wheat cultivar Thatcher. Seedling stage plant specimens examined under an Axio Imager M2 Zeiss Research epifluorescence microscope (a–h). a Germinated spore (S) 4 hpi, with branching germ tube (GT) and appressorium (AP) with lobes (L). b At 12 hpi, infection peg formed from appressorium and sub-stomatal vesicle (SSV) formation from infection peg. c At 12 hpi, Formation of infection hyphae (IH) from infection peg. d At 18 hpi, haustorial mother cell (HMC) produced a penetration peg that perforated through mesophyll cell wall and formed a haustorium in the host cell. e, f At 24 –96 hpi, increase number of haustorial mother cells, and colony development (C). g, h At 7 dpi, established colony (EC) and sporulation (SP)