Fig. 2
From: Rapid protocol for visualization of rust fungi structures using fluorochrome Uvitex 2B
Comparison of pre-staining incubation time. Leaves of seedling stage plants of cultivar Thatcher infected by Puccinia triticina were stained with fluorochrome Uvitex 2B and examined under an Axio Imager M2 Zeiss Research epifluorescence microscope. a Under-stained P. triticina colony in leaf tissue treated with a 5 min pre-staining incubation. b Brighter P. triticina colony after a 10 min pre-staining treatment in 0.1 M Tris–HCl. Established colony (EC)