Fig. 9
From: Protocol: an improved and universal procedure for whole-mount immunolocalization in plants
Simultaneous DNA and protein localization in Arabidospis roots. Four days old Arabidopsis thaliana seedlings were pre-cultured for 30 min in the presence of 15 μM BrdU in the dark. Plants were fixed in formaldehyde. Anti-PIN1 Guinea pig polyclonal primary antibody diluted 1:800 plus ALEXA Fluor ® 555 anti-Guinea pig as the secondary antibody diluted 1:800 (red color) and mouse BrdU primary antibody (Amersham, RPN202; http://www5.gelifesciences.com) diluted 1:150 (containing DNAse) plus ALEXA Fluor ® 488 goat anti-mouse IgG as secondary antibodies diluted 1:800 were used. Co-staining with DAPI visualizes nuclei (shown as artificial color in white). The projection of a 3D reconstruction of confocal images with IMARIS Software (Bitplane) is shown