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Fig. 6 | Plant Methods

Fig. 6

From: Protocol: an improved and universal procedure for whole-mount immunolocalization in plants

Fig. 6

Protein immunolocalization in different Triticum aestivum organs. Three days old wheat seedlings were fixed for 30 min in formaldehyde. Anti-PIN1 mouse monoclonal primary antibody (clone 10A7) diluted 1:50 and Alexa Fluor® 488 goat anti-mouse IgG as secondary antibody diluted 1:800 were used (shown in green color) (ae); anti-PIN2 Guinea pig primary antibody plus Goat anti-Guinea pig IgG Alexa Fluor® 647 conjugate as secondary antibody diluted 1:800 (shown in red color) (e) and anti-BIP2 (AS09 615) rabbit primary antibody plus Goat anti-rabbit IgG DyLight® 549 conjugate (AS09 642) as secondary antibody diluted 1:3000 (shown in red color) (f) were used. Co-staining with DAPI visualizes nuclei (blue). a leaf; b meristem; c coleoptile; df roots. Arrows point polarly located PIN1 and PIN2 proteins. Scale bar 20 µm

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