Fig. 5
From: Protocol: an improved and universal procedure for whole-mount immunolocalization in plants
PIN1 protein localization in Hedera helix stem, leaf and flowers. Explants were fixed in formaldehyde for 30 min. Anti-PIN1 mouse primary antibody (clone 10A7) diluted 1:50 plus Alexa Fluor® 488 goat anti-mouse as secondary antibody diluted 1:800 was used. Co-staining with DAPI visualizes nuclei (shown as artificial color in red). Scale bar 20 µm. a stem; b leaf; c flowers initial