Fig. 3
From: Protocol: an improved and universal procedure for whole-mount immunolocalization in plants
Auxin efflux carrier PIN1 localization in Arabidopsis flower organs. Whole siliques were fixed in formaldehyde and treated for 20 min with methanol. Anti-PIN1 mouse monoclonal primary antibody (clone 10A7), diluted 1:50 and ALEXA Fluor ® 488 goat anti-mouse IgG as secondary antibody (Invitrogen) diluted 1:800 were used. Co-staining with DAPI visualizes nuclei (blue). a Arabidopsis silique, stage 1; b Arabidopsis silique, stage 2; c Isolated ovules. Scale bar 20 µm