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Fig. 2 | Plant Methods

Fig. 2

From: Protocol: an improved and universal procedure for whole-mount immunolocalization in plants

Fig. 2

PIN1 protein localization in cotyledons and hypocotyls of Arabidopsis seedlings. Four days old seedlings were fixed for 20 min in methanol and subjected to the standard immunolocalization procedure as described. Anti-PIN1 mouse monoclonal primary antibody (clone 10A7), diluted 1: 50. ALEXA Fluor ® 488 conjugated goat anti-mouse IgG (Invitrogen) was used as secondary antibody diluted 1:800. Co-staining with DAPI visualized nuclei (red). a Cotyledon; b hypocotyl; Scale bar 20 µm

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