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Fig. 1 | Plant Methods

Fig. 1

From: The isolation of early nuclear endosperm of Oryza sativa to facilitate gene expression analysis and screening imprinted genes

Fig. 1

Early development of rice endosperm in hybrid of Nipponbare (♀) × 9311(♂). a Three hours after pollination (HAP), the central cell was not yet fertilized (arrowhead). b About 4 HAP, the central cell was fused with a sperm cell. The arrowhead indicates three nuclei in the central cell before nuclear fusion. c About 5 HAP, the three nuclei were fused and a primary endosperm nucleus was formed. d About 6 HAP, primary endosperm cells divided. The arrowhead indicates one of the two endosperm nuclei. e About 19 HAP, free endosperm nuclei (arrowheads) were distributed peripherally along the embryo sac. f About 46 HAP, free nuclei (arrowheads) constructed a complete network around the embryo sac. g About 67 HAP, endosperm cellularization was triggered. h Magnification of the boxed region in g to show cell wall formation (arrowheads). i About 96 HAP, the embryo sac was filled with endosperm nuclei. j Magnification of the squared part in i to show the cell wall (arrows). Bar 50 μm

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