Table 1 Troubleshooting guide
Steps | Problem | Possible reason | Solution |
|---|---|---|---|
20 | Anthrone solution not developing colour | Used 67 % sulphuric acid instead of conc. Sulphuric acid | Remake anthrone solution with conc. Sulphuric acid |
20 | Anthrone solution getting dark too quickly | Used sulphuric acid from an old bottle | Remake anthrone solution with conc. Sulphuric acid from a new bottle |
23 | OD620 values outside the standard curve range | Too much/too little sample used for anthrone assay | Use more/less amount of sulphuric acid treated sample |