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Fig. 5 | Plant Methods

Fig. 5

From: Protocol: a medium-throughput method for determination of cellulose content from single stem pieces of Arabidopsis thaliana

Fig. 5

Sample processing through various stages of acetic/nitric treatment (aj). One representative sample with a starting sample weight of 7 mg is shown (a). The wild type samples tend to be thicker and are much easier to track along the various steps. However, the irx mutant samples are comparatively thinner and they also tend to be more “wonky” making them relatively difficult to follow around during the aspiration steps. Also they go even more translucent than the wild samples making them more difficult to spot. So more care is needed in handling such samples. In step 13 (i), it is critical to push the samples down to the bottom of tube when they are still wet. Once dried at the bottom (j), they will be accessible to H2SO4 in step 16 (Fig. 3)

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